Particulate Gunylate Cyclase A (PGCA) from MyBioSource.com

Alternate nomenclature: Natriuretic peptide receptor A, NPR-A. Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP ate maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, sGC contains a heme group and binds NO that activates the enzyme, while particulate GC is stimulated by natriuretic peptides. In response to Gprotein couples receptor stimulation, the cGMP can be produced from GTP by either cytoplasmic, soluble guanylate cycl ase (sGC) are heterodimers (alpha and beta polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. Particulate GC (PGCs) have 7 different isoforms, PGC-A through PGC-G and are expressed in most tissues in isoform specific manner (See Table 1). There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Both PGC-A and PGC-B are phosphorylated at Serine residues in the KLD (2). Non-ionic detergents stimulated particulate guanylate cyclase activity in cerebral cortex of rat 8- to 12-fold while stimulation of soluble enzyme was 1.3- to 2.5-fold. Among various detergents (Duguch et. Al., 2002). It has been shown that a significant number hippocampal astrocytes (67%) contained both soluble and particulate guanylate cyclases in the same cell (2). The Anti-GC-A-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to PGC-A protein. The PGC-A-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The PGC-A antibodies label a 127-130 kDa protein in various tissues including brain, kidney and spleen. Anti-PGC-A-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at extra charge. MyBioSource also provides antibodies to other family members of the particulate GC (PGC-B, C, D, and G) and to adenylate cyclases (AC-1 through 9). MyBioSource employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). MyBioSource, will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available for blocking studies. Please enquire for their availability before ordering